Training manual for embryo transfer in cattle / by George E. Seidel and Sarah Moore Seidel.

Includes bibliographical references (pages 159-164).

Historic frame of reference -- Applications of embryo transfer -- Increase reproduction of females -- Circumvent infertility -- Import/export -- MOET programmes -- Twinning -- Embryo transfer as part of other biotechnology -- Managing donor and recipient herds -- Donor selection -- Selection of sires -- Management of donors -- Selection of recipients -- Management of recipients -- Oestrus detection -- Pregnancy diagnosis -- Managing pregnant recipients -- Superovulation -- Superovulatory treatments -- Insemination -- Recovery of embryos -- Non-surgical recovery of embryos -- Isolation of embryos -- Reflushing and prevention of multiple pregnancies in donors -- Maintaining embryos in vitro -- Storage conditions -- Media -- Macromolecular supplements -- Containers -- Pipettes -- Evaluation of embryos -- Embryological terminology -- Evaluation -- Transfer of embryos -- Surgical transfer -- Non-surgical transfer -- Loading straws -- Non-surgical transfer equipment -- Anaesthesia -- Transfer procedures -- Synchrony of productive cycles -- Methods of synchronizing oestrus.

(cont) Example of programming a herd for embryo transfer -- Cryopreservation of bovine embryos -- Splitting embryos -- Brief overview of emerging technologies -- Sexing embryos -- In vitro fertilization -- Chimeras -- Cloning by nuclear transplantation -- Embryonic stem-cells -- Transgenic animals -- Success rates of embryo transfer -- Measures of success -- Determinants of success -- Current production averages -- Costs of embryo transfer -- Embryo transfer and disease transmission -- Washing procedures for work areas, glassware and equipment -- Embryo handling area -- Glassware and metal equipment -- Plastic and other heat-labile equipment -- Solutions -- Siliconizing glassware -- Rinsing prior to use -- Record keeping -- Equipment and supplies -- Equipment, supplies, drugs and reagents -- Microscopes -- Freezing machines -- Quality control -- Records -- Culture medium -- Equipment and supplies -- Animals -- Semen handling and artificial insemination -- Embryo transfer procedures -- Training programmes -- Note to administrators -- Key ingredients for successful embryo transfer programmes -- Appropriate goals.