TY  - BOOK
AU  - Hamdoun,Amro
AU  - Foltz,Kathleen
TI  - Echinoderms
T2  - Methods in cell biology
AV  - QL381 HAM
U1  - 593.9 23
PY  - 2019///
CY  - Cambridge, San Diego, Oxford, London
PB  - Academic Press is an imprint of Elsevier
KW  - Echinodermata
KW  - SCIENCE / Life Sciences / Zoology / General
KW  - bisacsh
KW  - Electronic books
N1  - Academic; Front Cover; Echinoderms, Part B; Copyright; Contents; Contributors; Preface; Dedication; Coverblurb; 1: Fertilization; When sperm meets egg-Fifty years of surprises; Introduction; Cell biology in the late 1950s and early 1960s; My thesis problem circa 1959-control of the cell cycle; Control of the cell cycle circa today; How I became interested in fertilization; A wrong hypothesis directs me to a new area; My new field; Protein synthesis; A serendipitous finding leads to a new areacortical granules; The rest of the story on egg activation at fertilization; What are exciting areas for future research?References; Early and later studies on action potential and fertilization potential of echinoderm oocytes and Ca2+ re; References; My research career on (mainly) sea urchins; Getting hooked on a career in research; What led me into echinoderms?; How did I begin studying fertilization?; My most exciting finding; One important conceptual contribution of my lab; The most satisfying part of my career; Enter the new biology; Advice for people just beginning their careers; References; 2: Cytokenesis and embryology; Echinoderm eggs as a model for discoveries in cell biologyEarly cell biological work: Microscopy; Biochemistry of the cytoskeleton; Live cell imaging, biochemistry and cell manipulations; Super resolution imaging, live cell fluorescent imaging and mathematical modeling; Acknowledgments; References; Unlocking mechanisms of development through advances in tools; From hemoglobin to urchin spicules; 3: Genomics and morphogenesis; The causes of things; References; A personal history of the echinoderm genome sequencing; References; Section I: Methods for genome and transcriptome analysis; Chapter 1: Genomic resources for the study of echinoderm development and evolution1. Introduction; 2. Genome assembly; 3. Gene prediction and annotation; 4. Transcriptome assembly and quantitation; 5. Functional genomic analyses; 6. Other tools and resources; 6.1. BAC resources; 6.2. GRNs and BioTapestry; 7. Adding data to EchinoBase; 8. Conclusions; Acknowledgments; References; Chapter 2: Methods for the experimental and computational analysis of gene regulatory networks in sea urchins; 1. Introduction; 2. Experimental analysis of gene regulatory networks; 2.1. Identification of transcriptional regulators2.2. Identification of functional regulatory interactions by trans-perturbation; 2.2.1. Strategies for perturbation of regulatory factors; 2.2.2. Approaches for identification of target genes; 2.3. Identification of functional regulatory interaction by cis-perturbation; 2.3.1. Identification of active CRMs; 2.3.2. Identification of cis-regulatory inputs; 2.4. Physical interaction between transcription factors and DNA-binding site; 3. A strategy for data analysis and modeling of gene regulatory networks; Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK)
ER  -